Purification, characterization, and localization of an ATP diphosphohydrolase in porcine kidney.

Ectonucleoside triphosphate diphosphohydrolase 1/CD39, localized in neurons of human and porcine heart, modulates ATP-induced norepinephrine exocytosis.

Using a guinea pig heart synaptosomal preparation, we previously observed that norepinephrine (NE) exocytosis was attenuated by a blockade of P2X purinoceptors, potentiated by inhibition of ectonucleoside triphosphate diphosphohydrolase-1 (E-NTPDase1)/CD39, and reduced by soluble CD39, a recombinant form of human E-NTPDase1/CD39. This suggests that norepinephrine and ATP are coreleased upon dep...

Expression and Purification of the luciferase enzyme and in Vivo ATP Assay

Introduction: Gene expression and purification of luciferases from the firefly, Lampyris turkestanicus, and optimization of cellular ATP measurements were performed. Methods: cDNA encoding luciferases from Lampyris turkestanicus was transferred from pQE30 vector into pET28a expression vector and pLtu28 was built. Newly constructed vector was expressed in E. coli XL1 Blue and the recombinant l...

Purification and Characterization of Two Acid Phosphatases from Germinating Peanut (Arachis hypogaea) Seed

The maximum acid phosphatasic activity was detected in peanut seed at the 5th day of germination. At least, two acid phosphatases were purified by successive chromatography separations on DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, Sephacryl S-100 HR, and Phenyl-Sepharose HP to apparent homogeneity from five days old cotyledon of peanut after germination. These isoenzymes, designated peanut cotyl...

PURIFICATION, PAHflAL CHARACTERIZATION OF RAT KIDNEY HYALURONIC ACID-BINDING PROTEIN AND ITS LOCALIZATION ON THE CELL

Structure and function of renin binding protein.

Renin binding protein (RnBP) is a proteinous renin inhibitor. This protein binds to renin forming a protein complex, so-called high molecular weight (HMW) renin. The protein complex has been confirmed by purification and characterization of HMW renin [1] and RnBP from porcine kidney [2, 31. Recently, cDNA clones encoding RnBPs were isolated from porcine, human and rat kidney cDNA libraries [4, ...